{"id":80751,"date":"2021-03-11T15:58:20","date_gmt":"2021-03-11T18:58:20","guid":{"rendered":"https:\/\/folhapopular.info\/?p=80751"},"modified":"2021-03-11T15:59:00","modified_gmt":"2021-03-11T18:59:00","slug":"equipe-da-univates-identifica-mutacoes-no-genoma-do-sars-cov-2-virus-causador-da-covid-19","status":"publish","type":"post","link":"https:\/\/folhapopular.info\/index.php\/2021\/03\/11\/equipe-da-univates-identifica-mutacoes-no-genoma-do-sars-cov-2-virus-causador-da-covid-19\/","title":{"rendered":"Equipe da Univates identifica muta\u00e7\u00f5es no genoma do SARS-CoV-2, v\u00edrus causador da Covid-19"},"content":{"rendered":"\n

Um grupo de pesquisadores da Universidade do Vale do Taquari – Univates liderados pelo professor doutor Luis Fernando Saraiva Macedo Timmers realizou a an\u00e1lise de 627 genomas do SARS-CoV-2, v\u00edrus causador da Covid-19, isolados de indiv\u00edduos de diferentes regi\u00f5es do Brasil. <\/p>\n\n\n\n

Durante o trabalho, os pesquisadores combinaram an\u00e1lises de gen\u00f4mica e bioinform\u00e1tica estrutural para avaliar a presen\u00e7a de muta\u00e7\u00f5es no genoma de SARS-CoV-2, com o objetivo de demonstrar quais s\u00e3o e onde, na estrutura do v\u00edrus, est\u00e3o as muta\u00e7\u00f5es mais prevalentes. <\/p>\n\n\n\n

O estudo expande o conhecimento sobre a intera\u00e7\u00e3o do v\u00edrus da Covid-19 com o organismo humano. <\/p>\n\n\n\n

Objetivos do trabalho <\/strong><\/p>\n\n\n\n

O principal objetivo do estudo foi avaliar a presen\u00e7a de muta\u00e7\u00f5es no genoma do SARS-CoV-2 isolados em diferentes regi\u00f5es do Brasil. \u201cN\u00f3s vemos muitas not\u00edcias de muta\u00e7\u00f5es sendo notificadas em outros lugares do mundo e, \u00e0 altura, havia poucas informa\u00e7\u00f5es sobre o Brasil. J\u00e1 existem mudan\u00e7as no genoma de SARS-CoV-2 circulando por aqui? Em caso positivo, quais s\u00e3o os genes que apresentam as maiores taxas de muta\u00e7\u00f5es?\u201d, essas foram perguntas que guiaram o estudo, diz Timmers. <\/p>\n\n\n\n

Os dados para este estudo foram obtidos por meio do banco de dados p\u00fablico GISAID<\/a>.<\/p>\n\n\n\n

\"\"
Esquema que demonstra as etapas do estudo \/ Cr\u00e9dito da foto: Divulga\u00e7\u00e3o<\/figcaption><\/figure>\n\n\n\n

Resultados<\/strong><\/p>\n\n\n\n

Os resultados demonstraram que grande parte das muta\u00e7\u00f5es foram observadas em quatro prote\u00ednas virais: (i) Spike<\/em>, (ii) nucleocaps\u00eddeo, (iii) ORF3a e (iv) ORF6. Essas prote\u00ednas est\u00e3o envolvidas em diferentes est\u00e1gios do ciclo viral, assim como no processo de  intera\u00e7\u00e3o com as c\u00e9lulas humanas. <\/p>\n\n\n\n

Um ponto que chamou a aten\u00e7\u00e3o dos pesquisadores foi a presen\u00e7a de diferentes muta\u00e7\u00f5es na regi\u00e3o da prote\u00edna Spike<\/em> – respons\u00e1vel por reconhecer a c\u00e9lula humana e iniciar o processo de infec\u00e7\u00e3o pelo v\u00edrus. <\/p>\n\n\n\n

Para chegar \u00e0s respostas a equipe combinou diversas t\u00e9cnicas computacionais como  a bioinform\u00e1tica estrutural e a gen\u00f4mica comparativa. Desta forma, foi poss\u00edvel observar que as muta\u00e7\u00f5es estavam acontecendo, sim. <\/p>\n\n\n\n

A an\u00e1lise estrutural realizada durante a pesquisa trouxe luz \u00e0s posi\u00e7\u00f5es em que as altera\u00e7\u00f5es acontecem nas prote\u00ednas virais, o que pode auxiliar estudos sobre a descoberta de medicamentos baseados em estrutura (structure-based drug discovery<\/em>), no desenvolvimento de vacinas, assim como, o impacto dessas varia\u00e7\u00f5es na efic\u00e1cia das vacinas atuais. <\/p>\n\n\n\n

\u201cUma vez identificadas as muta\u00e7\u00f5es, observamos onde elas estavam localizadas na estrutura das prote\u00ednas virais\u201d, diz o docente.  <\/p>\n\n\n\n

Os dados dos genomas estudados est\u00e3o em bancos de dados p\u00fablicos, de onde as amostras coletadas vieram e nas quais estudos como o da Univates ainda n\u00e3o haviam sido realizados. <\/p>\n\n\n\n

Timmers defende a import\u00e2ncia de se conhecer a exist\u00eancia de muta\u00e7\u00f5es em curso no v\u00edrus, quais s\u00e3o e onde est\u00e3o localizadas. <\/p>\n\n\n\n

\u201cEspecialmente neste momento de desenvolvimento de vacinas \u00e9 essencial que se conhe\u00e7a mais\u201d. Casos em que muta\u00e7\u00f5es influenciam os efeitos da vacina j\u00e1 foram verificados. <\/p>\n\n\n\n

\u201cA neutraliza\u00e7\u00e3o viral induzida pela vacina da Oxford\/AstraZeneca, parece ser significativamente reduzida contra a variante B.1.351, identificada na \u00c1frica do Sul\u201d, lembra o docente. <\/p>\n\n\n\n

Quanto mais o v\u00edrus circula, maior as chances de ocorrerem muta\u00e7\u00f5es no genoma viral. \u201cDesta forma, mesmo com o in\u00edcio do processo de vacina\u00e7\u00e3o, ainda s\u00e3o extremamente importantes manter as medidas de higieniza\u00e7\u00e3o, uso de m\u00e1scara e distanciamento social\u201d, finaliza o pesquisador.<\/p>\n\n\n\n

Parceria<\/strong><\/p>\n\n\n\n

Timmers explica que a investiga\u00e7\u00e3o foi delineada na Institui\u00e7\u00e3o, numa parceria dele com os professores doutor. Jo\u00e3o Ant\u00f4nio P\u00eagas Henriques e a doutora M\u00e1rcia Goettert – que, no momento, est\u00e1 na Universidade de T\u00fcbingen, na Alemanha, pesquisando o desenvolvimento de novos testes para detec\u00e7\u00e3o de SARS-CoV-2. <\/p>\n\n\n\n

Al\u00e9m dos professores da Univates, a pesquisa contou com a colabora\u00e7\u00e3o de mais seis institui\u00e7\u00f5es: a Universidade Federal do Rio Grande do Sul (UFRGS), a Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA), a Universidade Federal do Rio de Janeiro (UFRJ), a Universidade Federal de Pelotas (UFPel), a Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS) e a Universidade de T\u00fcbingen. <\/p>\n\n\n\n

O artigo est\u00e1 publicado no formato preprint – ou seja, pr\u00e9-publicado -, na revista ChemRxiv. Aqui http:\/\/bit.ly\/pesquisa-genoma-univates<\/a> \u00e9 poss\u00edvel acessar o conte\u00fado integral da publica\u00e7\u00e3o da Univates.<\/p>\n","protected":false},"excerpt":{"rendered":"

Um grupo de pesquisadores da Universidade do Vale do Taquari – Univates liderados pelo professor doutor Luis Fernando Saraiva Macedo Timmers realizou a an\u00e1lise de 627 genomas do SARS-CoV-2, v\u00edrus causador da Covid-19, isolados de indiv\u00edduos de diferentes regi\u00f5es do Brasil.  Durante o trabalho, os pesquisadores combinaram an\u00e1lises de gen\u00f4mica e bioinform\u00e1tica estrutural para avaliar […]<\/p>\n","protected":false},"author":8,"featured_media":80755,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pmpro_default_level":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[2,3],"tags":[5707,83],"class_list":["post-80751","post","type-post","status-publish","format-standard","has-post-thumbnail","category-featured","category-geral","tag-covid-19","tag-univates","pmpro-has-access"],"yoast_head":"\nEquipe da Univates identifica muta\u00e7\u00f5es no genoma do SARS-CoV-2, v\u00edrus causador da Covid-19 - 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